abstract：:Coupling of ATP-generating with ATP-consuming processes is an essential component in the cardiac bioenergetics responsible for optimal myocardial function. Although a number of enzymatic systems have been implicated in securing proper intracellular energy communication, their integrative response in a failing myocardi...

journal_title：Molecular and cellular biochemistry

authors： Dzeja PP,Pucar D,Redfield MM,Burnett JC,Terzic A

更新日期：1999-11-01 00:00:00

abstract：:This study examined the role of the protein kinase C (PKC) signalling pathway in the regulation of expression of human complement factor I (CFI) gene. The production of CFI by Hep G2 cells was enhanced in a dose- and time-dependent fashion by 12-O-tetradecanoyl-1,2-phorbol 13-acetate (TPA), a potent PKC activator. 4Al...

journal_title：Molecular and cellular biochemistry

authors： Minta J,Fung M

更新日期：1999-11-01 00:00:00

abstract：:Nitric oxide (NO) is a messenger molecule which regulates many physiological functions like immunity, vascular tone and serves as a neurotransmitter. Although it is known to participate in healing process, its role in collagen synthesis is not clear. Therefore, the present investigation was done to study the role of N...

journal_title：Molecular and cellular biochemistry

authors： Shukla A,Rasik AM,Shankar R

更新日期：1999-10-01 00:00:00

abstract：:The evolution of pyruvate carboxylase has been studied in rat liver and kidney during perinatal development. The pyruvate carboxylase activity, amount of enzyme and mRNA levels have been assayed from 2 days before delivery to weaning. In liver, there is a peak of activity and amount of enzyme 24 h before delivery and ...

journal_title：Molecular and cellular biochemistry

authors： Salto R,Girón MD,del Mar Sola M,Vargas AM

更新日期：1999-10-01 00:00:00

abstract：:The human breast cancer cell line MCF7 does not express heart-type fatty acid binding protein (H-FABP), a marker protein for differentiated mammary gland. MCF7 cells transfected with the bovine H-FABP cDNA expressed the corresponding protein and were characterized by growth inhibition and lower tumorgenicity in nude m...

journal_title：Molecular and cellular biochemistry

authors： Buhlmann C,Börchers T,Pollak M,Spener F

更新日期：1999-09-01 00:00:00

abstract：:In myocardial cells (MCs), endothelin-1 (ET-1) exerts various effects such as hypertrophy, and causes cellular injury. Long-term treatment with an endothelin-A (ET(A)) receptor antagonist improves the survival of rats with heart failure, suggesting that myocardial endothelin system contributes to the progression of he...

journal_title：Molecular and cellular biochemistry

authors： Ueyama T,Kawashima S,Sakoda T,Rikitake Y,Ishida T,Kawai M,Namiki M,Yokoyama M

更新日期：1999-09-01 00:00:00

abstract：:Brain membrane lipid fatty acid composition and consequently membrane fluidity change with increasing age. Intracellular fatty acid binding proteins (FABPs) such as heart H-FABP and the brain specific B-FABP, detected by immunoblotting of brain tissue, are thought to be involved in fatty acid uptake, metabolism, and d...

journal_title：Molecular and cellular biochemistry

authors： Pu L,Igbavboa U,Wood WG,Roths JB,Kier AB,Spener F,Schroeder F

更新日期：1999-08-01 00:00:00

abstract：:Female rats at weaning (30 days age) were maintained on vitamin E-deficient diet for 70 days. The vitamin E-deficient and control animals were sacrificed on 100 days of age. To study recovery a group of animals were supplemented with normal diet for last 25 days after initial 45 days of deficient diet or vice versa. T...

journal_title：Molecular and cellular biochemistry

authors： Das P,Chowdhury M

更新日期：1999-08-01 00:00:00

abstract：:The effect of anti-regucalcin monoclonal antibody on neutral phoshatase activity in rat liver cytosol was investigated. Phosphotyrosine, phosphoserine, and phosphothreonine were used as the substrate toward phosphatase assay. Liver cytosolic phosphatase activity with three phosphoaminoacids was significantly increased...

journal_title：Molecular and cellular biochemistry

authors： Omura M,Yamaguchi M

更新日期：1999-07-01 00:00:00

abstract：:Young female Zucker fa/fa rats of 370-430 g were implanted with osmotic minipumps releasing 3.5 micromol/day-kg of estrone oleate in liposomes (Merlin-2) into the bloodstream for up to 14 days. Merlin-2 induced a sustained loss of appetite, and a decrease in body weight of 3.5%, which contrasts with the 8.2% increase ...

journal_title：Molecular and cellular biochemistry

authors： Adán C,Grasa MM,Cabot C,Esteve M,Vilà R,Masanés R,Estruch J,Fernández-López JA,Remesar X,Alemany M

更新日期：1999-07-01 00:00:00

abstract：:This study was designed to investigate the alterations in the levels of various proteases such as angiotensin converting enzyme (ACE), kallikrein, aminopeptidases, urokinase and plasmin in serum-heart and kidney and to find out whether the changes in the levels of these enzymes could explain the pathogeneses of hypert...

journal_title：Molecular and cellular biochemistry

authors： Rajashree S,Puvanakrishnan R

更新日期：1999-07-01 00:00:00

abstract：:Glutathione plays a central role in the maintenance of tissue antioxidant defenses and in the regulation of redox sensitive signal transduction. In muscle cells, the level and redox status of GSH regulates activity of the redox sensitive transcription factor NF-kappaB. Physical exercise may cause oxidation of GSH in t...

journal_title：Molecular and cellular biochemistry

authors： Sen CK

更新日期：1999-06-01 00:00:00

abstract：:Stress genes can be ascribed to have been generated by the organism for their intrinsic urge to survive against the changing environmental odds, during the evolutionary process. This concept has been supported by a large number of reports describing individual types of phenomena. These have been reconciled and globali...

journal_title：Molecular and cellular biochemistry

authors： Ray PK

更新日期：1999-06-01 00:00:00

abstract：:Experiments were conducted to study the functional implication of a mannose-binding glycoprotein found in the day 4 post coital (p.c.) rat uterus, using a mono-specific polyclonal antibody raised against the glycoprotein. Western Blot and immunohistochemical techniques were employed to study the distribution of the gl...

journal_title：Molecular and cellular biochemistry

authors： Mehta M,Chowdhury M

更新日期：1999-05-01 00:00:00

abstract：:The effects of chloroperoxidase [EC 1.1.1.10] and hydrogen peroxide on the viabilities of quiescent and germinating conidiospores of an aflatoxigenic fungus, Aspergillus flavus, were determined. Hydrogen peroxide was found moderately lethal and chloroperoxidase produced a 30-fold increase in the lethality of hydrogen ...

journal_title：Molecular and cellular biochemistry

authors： Jacks TJ,De Lucca AJ,Morris NM

更新日期：1999-05-01 00:00:00

abstract：:We examined the Hepatitis C virus (HCV) genome in the myocardium and liver obtained at autopsy from seven patients with HCV-positive liver cirrhosis and hepatocellular carcinoma (HCC) by in situ hybridization and histopathological studies. The HCV virus genome was detected in the myocardium of one patient as well as i...

journal_title：Molecular and cellular biochemistry

authors： Takeda A,Sakata A,Takeda N

更新日期：1999-05-01 00:00:00

abstract：:The natriuretic peptide receptor-A (NPR-A) is involved in blood pressure and body fluid regulation in order to help maintain cardiovascular homeostasis. It has been shown that these biological effects are mediated through the natriuretic peptide family of hormones, which bind NPR-A according to the rank order ANP>BNP>...

journal_title：Molecular and cellular biochemistry

authors： Marquis M,Fenrick R,Pedro L,Bouvier M,De Léan A

更新日期：1999-04-01 00:00:00

abstract：:To determine the role of 'translocation' vs. 'activation' of Glut1 in the stimulation of glucose transport in response to inhibition of oxidative phosphorylation, we measured the abundance of myc-tagged Glut1 in plasma membrane of stably transfected Clone 9 cells, a rat liver cell line expressing only the Glut1 isofor...

journal_title：Molecular and cellular biochemistry

authors： Koseoglu MH,Beigi FI

更新日期：1999-04-01 00:00:00

abstract：:To investigate whether the action potential duration (APD) or resting tension was dependent on global ATP content, and whether they were preferentially dependent on glycolytic ATP, APD and resting tension were measured under various metabolic inhibition with corresponding measurement of ATP content in guinea pig ventr...

journal_title：Molecular and cellular biochemistry

authors： Hayashi H,Terada H,McDonald TF

更新日期：1999-04-01 00:00:00

abstract：:Cellular adaptation to hypoxia involves regulation of specific genes such as vascular endothelial growth factor (VEGF), erythropoietin (EPO) and hypoxia inducible factor (HIF)-1 . In this study, we have evaluated the protective effect of picroliv (a purified iridoid glycoside fraction from roots of Picrorhiza kurrooa ...

journal_title：Molecular and cellular biochemistry

authors： Gaddipati JP,Madhavan S,Sidhu GS,Singh AK,Seth P,Maheshwari RK

更新日期：1999-04-01 00:00:00

abstract：:Studies presented here show that cellular NAD, which we hypothesize to be the relevant biomarker of niacin status, is significantly lower in humans than in the commonly studied animal models of carcinogenesis. We show that nicotinamide and the resulting cellular NAD concentration modulate expression of the tumor suppr...

journal_title：Molecular and cellular biochemistry

authors： Jacobson EL,Shieh WM,Huang AC

更新日期：1999-03-01 00:00:00

abstract：:We have focused on the roles of PARP and poly(ADP-ribosyl)ation early in apoptosis, as well as during the early stages of differentiation-linked DNA replication. In both nuclear processes, a transient burst of PAR synthesis and PARP expression occurs early, prior to internucleosomal DNA cleavage before commitment to a...

journal_title：Molecular and cellular biochemistry

authors： Simbulan-Rosenthal CM,Rosenthal DS,Iyer S,Boulares H,Smulson ME

更新日期：1999-03-01 00:00:00

abstract：:Parasitic nematodes have recently been found to produce proteins which represent two new classes of fatty acid and retinoid binding protein. The first is the nematode polyprotein allergens/antigens (NPAs) which, as their name suggests, are synthesised as large polyproteins which are subsequently cleaved at regularly s...

journal_title：Molecular and cellular biochemistry

authors： McDermott L,Cooper A,Kennedy MW

更新日期：1999-02-01 00:00:00

abstract：:Plant cells contain lipid-transfer proteins (LTPs) able to transfer phospholipids between membranes in vitro. Plant LTPs share in common structural and functional features. Recent structural studies carried out by NMR and X-ray crystallography on an LTP isolated from maize seeds have showed that this protein involves ...

journal_title：Molecular and cellular biochemistry

authors： Guerbette F,Grosbois M,Jolliot-Croquin A,Kader JC,Zachowski A

更新日期：1999-02-01 00:00:00

abstract：:Synthetic peptides reproducing the amino and carboxyl terminal region of CK2beta subunit have been analyzed for their ability to mimic different properties of full length beta subunit. Peptide beta[1-77], containing both the autophosphorylation site and the down-regulatory domain 55-64, is readily phosphorylated by al...

journal_title：Molecular and cellular biochemistry

authors： Meggio F,Marin O,Sarno S,Pinna LA

更新日期：1999-01-01 00:00:00

abstract：:BTF3, initially discovered as a factor required for transcription inititation of RNA polymerase II, is expressed in two isoforms, termed a and b. BTF3b, the transcriptionally inactive isoform, was identified as an interaction partner of protein kinase CK2 subunit beta employing the interaction trap system for screenin...

journal_title：Molecular and cellular biochemistry

authors： Grein S,Pyerin W

更新日期：1999-01-01 00:00:00

abstract：:Despite its wide range of known substrates, the signaling function of protein kinase CK2 is still enigmatic. Mounting evidence suggests that CK2alpha, the catalytic subunit of holoenzymic CK2, may exist free of its usual regulatory partner CK2beta, raising the possibility that 'free' CK2alpha has regulation and functi...

journal_title：Molecular and cellular biochemistry

authors： Lebrin F,Bianchini L,Rabilloud T,Chambaz EM,Goldberg Y

更新日期：1999-01-01 00:00:00

abstract：:We found out a new protein from natural actomyosin prepared from adductor muscle of Hokki clam, bivalve shell. We isolated this protein and determined some properties. It has a large molecular weight (230 kDa) and the star diagram of amino acid composition was very similar to that of paramyosin (110 kDa). When this pr...

journal_title：Molecular and cellular biochemistry

authors： Yazawa Y,Kamidochi M

更新日期：1999-01-01 00:00:00

abstract：:Cofilin is an actin-binding protein of low molecular weight which is widely distributed in eukaryotes and is deeply involved in the dynamics of actin assembly in the cytoplasm. The actin-binding ability of cofilin is inhibited by inositol phosphates (PIP2), and the PIP2- and actin-binding site(s) has been localized in...

journal_title：Molecular and cellular biochemistry

authors： Kusano K,Abe H,Obinata T

更新日期：1999-01-01 00:00:00

abstract：:Gram negative sepsis and septic shock continue to be a major medical problem, with a complex physiopathology and it is associated with high mortality. Although secretion of cytokines such as tumor necrosis factor-alpha by macrophages is the principal host mediator of septic shock, other characteristic functions of mac...

journal_title：Molecular and cellular biochemistry

authors： Víctor VM,Miñano M,Guayerbas N,Del Río M,Medina S,De la Fuente M

更新日期：1998-12-01 00:00:00

abstract：:Rat pheochromocytoma PC12 cells have been treated with nerve growth factor (NGF) at final concentrations of 2, 4, 8, and 16 ng/ml, and then were exposed to 60-Hz, sinusoidal magnetic fields (MF) of 12.5, 25, 50, and 100 microT (rms) for 30 min. Transcript levels for both c-fos and glyceraldehyde-3 -phosphate dehydroge...

journal_title：Molecular and cellular biochemistry

authors： Campbell-Beachler M,Ishida-Jones T,Haggren W,Phillips JL

更新日期：1998-12-01 00:00:00

abstract：:Full-length hexokinase (HK; ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1), a truncate form of the enzyme lacking the first 11 amino acids (HK-11aa) and the 50 kDa C-terminal half ('mini'-HK) containing the catalytic domain, were overexpressed and purified to homogeneity to investigate the influence of the N-terminal...

journal_title：Molecular and cellular biochemistry

authors： Bianchi M,Serafini G,Bartolucci E,Giammarini C,Magnani M

更新日期：1998-12-01 00:00:00

abstract：:During fetal life, myocardial ATP is derived predominantly from glycolysis and lactate oxidation. Following birth, a rapid maturational increase in fatty acid oxidation occurs along with a decline in glycolytic and lactate oxidative rates, thus changing the major source of myocardial ATP production. This shift in ener...

journal_title：Molecular and cellular biochemistry

authors： Makinde AO,Kantor PF,Lopaschuk GD

更新日期：1998-11-01 00:00:00

abstract：:Oxidized LDL is highly atherogenic as it stimulates macrophage cholesterol accumulation and foam cell formation, it is cytotoxic to cells of the arterial wall and it stimulates inflammatory and thrombotic processes. LDL oxidation can lead to its subsequent aggregation, which further increases cellular cholesterol accu...

journal_title：Molecular and cellular biochemistry

authors： Aviram M,Fuhrman B

更新日期：1998-11-01 00:00:00

abstract：:Incubation of Clone 9 cells, a nontransformed rat liver cell line, in the presence of 5 mM azide results in an induction of GLUT1 mRNA which becomes detectable after approximately 3 h of continuous exposure to the agent. In examining the role of on-going protein synthesis in this response, we found that: (i) the induc...

journal_title：Molecular and cellular biochemistry

authors： Behrooz A,Ismail-Beigi F

更新日期：1998-10-01 00:00:00

abstract：:Inhibition of synthesis of estradiol 17beta by the addition of inhibitors of aromatase, a key enzyme in the biosynthesis of estradiol 17beta, or addition of tamoxifen - an estrogen receptor antagonist, to human placental minces resulted in an increase in the level of LDL-receptor mRNA. This increase could be blocked b...

journal_title：Molecular and cellular biochemistry

authors： Shanker YG,Shetty UP,Rao AJ

更新日期：1998-10-01 00:00:00

abstract：:The heptahelical lutropin receptor (LHR) signals primarily via the Gs-adenylyl cyclase pathway and undergoes ligand-mediated receptor desensitization and internalization. A loss-of-function rat LHR mutant was recently described in which a single amino acid residue replacement in exoloop 3, K583E, had no effect on huma...

journal_title：Molecular and cellular biochemistry

authors： Bhowmick N,Narayan P,Puett D

更新日期：1998-10-01 00:00:00

abstract：UNLABELLED:Inhibition of Na+/H+ exchange with amiloride analogues has been shown to provide functional protection during ischemia and reperfusion and to reduce infarct size in isolated rat hearts. In rat hearts, treatment with ethylisopropyl-amiloride (EIPA, a selective Na+/H- exchange inhibitor) was additive to the pr...

journal_title：Molecular and cellular biochemistry

authors： Munch-Ellingsen J,Løkebø JE,Bugge E,Ytrehus K

更新日期：1998-09-01 00:00:00

abstract：:In view of the accumulation of H2O2 in the myocardium due to ischemia-reperfusion and changes in beta-adrenoceptor mechanisms in the ischemic-reperfused heart, we investigated the effects of H2O2 on the beta-adrenoceptor, G-protein and adenylyl cyclase complex. Rat hearts were perfused with 1 mM H2O2 for 10 min before...

journal_title：Molecular and cellular biochemistry

authors： Persad S,Panagia V,Dhalla NS

更新日期：1998-09-01 00:00:00

abstract：:When severe ischemia, such as that resulting from a sudden and complete coronary artery occlusion, is prolonged for more than 20-40 min, myocardial infarction develops, and there is irreversible loss of contractile function. When myocardial ischemia is less severe but nevertheless prolonged, the myocardium is dysfunct...

journal_title：Molecular and cellular biochemistry

authors： Heusch G,Schulz R

更新日期：1998-09-01 00:00:00